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Investigation Areas Receptor-binding and selectivity screening (in vitro) Pathway signaling and downstream mediator assays Solubility, vehicle selection, and excipient compatibility Stability studies (temperature/light/oxidation) Method development and calibration standards Handling Requirements Use calibrated balance and sterile technique as applicable Minimize moisture exposure

With the continuous emergence of novel biologics and small molecules, clinical decision-making must integrate disease conditions, medical history, comorbidities, and safety considerations to develop precise sequential strategies [330, 331]

In the 1990s, Balla et al

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