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The old RPMI-1640 medium containing the mixture was aspirated after a 48-h incubation, the dishes were washed with PBS, and 10 ml of fresh RPMI-1640 medium supplemented with 10% FBS was added and incubated for another 24 h (a total of 72-h incubations after starting the siRNA transfections)

[PubMed: 12956958] 50

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8 However, in the isolated perfused rat kidney, Ang A induced dose-dependent vasoconstriction, which was abolished by AT 1 receptor blockade, but not by the AT 2 receptor antagonist PD123319 (ref