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5 Discussion 5.1 Discussion of experimental methodology In validation experiments, joint cavity effusion and tophi showed complex and distinct protein compositions

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The western blot was performed using a GPX4 antibody (Abcam, [EPNCIR144], ab125066) (1:1000), -actin antibody (Abcam, ab8227) (1:5000) for loading control, and goat anti-rabbit IgG (H + L) secondary antibody, HRP (ThermoFisher Scientific, 31460) (1:3000)

Ethics statement The studies involving human participants were reviewed and approved by CAMH Research Ethics Board