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Comprehensive protocols built around individual lab work and goals

doi: 10.1016/j.it.2006.11.005 167 HaaseHRinkL

doi: 10.1152/ajpendo.00656.2013 57 XieZAitkenDLiuMLeiGJonesGCicuttiniFet al

We identified six biophysical properties that can be predicted from a sequence using the ProtParam module ( from the biopython Python package 45 : (1) the isoelectric point in neutral pH, (2) GRAVY (grand average of hydropathy) 46 , (3) the instability index 47 , (4) aromaticity, (5) the molar extinction coefficient and (6) molecular weight

2 Materials and methods 2.1 Animals Four-month-old male and female APOE3 and APOE4 targeted replacement mice ( n = 8 per genotype/sex combination) from Taconic Biosciences (Germantown, NY) were used for all analyses