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Cells were subsequently incubated with an Alexa Fluor-conjugated secondary antibody (1:1000, A-11012, Thermo Fisher Scientific) for 30 minutes at room temperature, protected from light

This dysregulated cholesterol metabolism affects immune cell function, inducing mitochondrial dysfunction in CD8 + T cells and MDSCs ( BOS complex subunit transmembrane protein 147 (TMEM147) is upregulated in hepatocellular carcinoma (HCC) cells

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