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Aliquots of compound solution were added to cells (final concentrations were 20 M forskolin, and 0.4% DMSO) and incubated at 37 C and 5% CO 2 for 30 min

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The secretion of inflammatory cytokines, such as tumor necrosis factor (TNF)-a, would lead to neuroinflammation and migraine episodes by increasing the cellular permeability and interactions

while nuclear -catenin expression was more predominant in MiT variant (8/20: 40%) and CK19 positivity was most frequent in PG pattern (2/2: 100%)