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In brief, standard solution (0, 15, 30, 60, 90, 120 and 150 mg/l creatinine in dH 2 0) or serum was added to a working solution (0.1 g picric acid in 50 ml dH 2 0 and 50 ml 0.4 NaOH in 4 C) (1:10), incubated at RT for 15 min and measured at 492 nm

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