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10.3389/fgene.2022.1051395 Front

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Numbers above the gels correspond to the fraction numbers from the elution profiles shown in panel A

88 (18), e0099122

To assess the presence of potential off-target effects of the CRISPR/Cas9 sgRNA sequences used to generate the Mettl9 KO cell lines, we predicted all putative off-target sites using Cas-OFFinder 154 , allowing up to five mismatches across the 20-base target sequence (which is the highest value permitted by the program)