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3D spheroids were fixed with 4% paraformaldehyde/PBS for 45 min at 4 C, washed once with 0.1% Tween-20 in PBS, and suspended in wash buffer (SWB: 2% BSA, 0.1% Triton X-100, 0.025% SDS, 1 PBS)

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As long-chain ACs are excreted and metabolized in the kidney, it might be the reason for the increase of serum AC levels in male uremic HD patients

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