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Several studies have suggested that the epithelial barrier is compromised in rodents exposed to various forms of PM ( 10 led to increased epithelial permeability in rat lungs, as indicated by enhanced total protein levels in bronchoalveolar lavage (BAL) fluid, which was associated with cell toxicity and inflammation ( 2.5 , these particles reduced the expression of E-cadherin in the lung tissue, which was linked to an increase in inflammatory cytokines in the BAL fluid ( 4 Inflammatory effects of PM on airways The vast majority of the literature suggests increases in inflammatory changes in the airways and lungs following PM exposure ( Figure 1 )

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Flow cytometry Bacteria labelled with either 488 or Cy5 were washed and resuspended in 100 l 1 PBS

BPC-157: Sequence: Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val | Molecular Formula: CHNO | MW: 1,419.5 g/mol | CAS: 137525-51-0 TB-500 (T4 fragment): Sequence: Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser | MW: ~4,963 g/mol | CAS: 77591-33-4