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Gene specific cDNAs were amplified by SYBR Green Pro Taq HS premixed qPCR kit (AG11701, Accurate Biology, China) and the corresponding expression levels were detected by the LightCycler480 PCR system (Roche)

For complex formation we incubated the hTrxR1 mutants with NADPH and the respective hTrx1 mutants

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This suggests that the benefits of treatment are sustained over time , offering long-term improvements in areas like speech, behavior, focus, and emotional regulation