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2 10 The unsaturated acyl chains in membrane phospholipids and cholesterol in membranes, among biomolecules, are highly susceptible to pathologic free radical damage for the following reasons 16 : First, the inherent structure of polyunsaturated fatty acid chains (polyunsaturated acyl chains are normally unconjugated, and the alpha-methylenic carbons between carbons with double bonds have allylic hydrogen that can readily enter into free radical reaction

Cells were cultured in Minimum Essential Medium supplemented with 10% (v/v) fetal bovine serum, 2 mmol/L l-glutamine, 1 mmol/L sodium pyruvate, 10 mmol/L HEPES, and 50 mol/L penicillin/streptomycin, and maintained at 37 C in a sterile humidified atmosphere with 5% CO 2

[8] Key biochemicals [edit] Most of the structures that make up animals, plants and microbes are made from four basic classes of molecules: amino acids, carbohydrates, nucleic acid and lipids (often called fats)

The iron chelator deferiprone was reported not to impact C

10.1016/j.cmet.2016.12.022 [DOI] [PMC free article] [PubMed] [Google Scholar] 231.Batch JT, Lamsal SP, Adkins M, Sultan S, Ramirez MN