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In their study divided Piezo1 channels into three functional parts based on structure and function: (1) the ion-conducting pore part (the C-terminus)

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After 8 h, cells were harvested in Passive Lysis Buffer (Promega Corp., Madison, WI) and the luciferase activity was measured by the Nano Dual-luciferase report assay System (Promega Corp., Madison, WI)