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RNA-binding elements Although charge-reversal mutations within RBE2 and RBE3 did not individually impact rescue of miR-7 degradation in ZSWIM8-deficient cells, combined mutation of all three RBEs exacerbated the defect caused by mutating RBE1, leading to severe loss of both TDMD in cells and polyubiquitylation in vitro (Fig

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Additionally, these GSTs are also largely distributed among kingdoms since they are found in mammals, nematodes, insects, and trypanosomatids but not fungi (Table 2)

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USA 120 , e2301360120 (2023)