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Glycine, usually protective, may paradoxically increase oxidative stress

Conversely, free radicals can increase the concentration of glutamate in the synaptic cleft by the release of glutamate or blockade of its re-uptake

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After treatment with the blocking solution (0.3% Triton-X and 3% BSA in 0.01 M PBS) to block non-specific staining and increase permeability to antibodies, the brain sections were incubated with anti-c-Fos rabbit polyclonal antibodies (abcam) in the blocking solution at 4 C overnight