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The plate was placed into a 37 C incubator for 2 hours before 2 L of a 2.5 M SK-575 (MedChem Express) solution in RPMI with 10% FBS was added to each well, and the plate was again placed on an orbital shaker for 15 seconds (final concentration of SK-575 is 250 nM, control wells are treated with an equal volume of DMSO in RPMI with 10% FBS)

This mechanism is vital for longitudinal studies examining progressive metabolic adaptation, particularly in age-related metabolic decline scenarios where pituitary efficiency naturally wanes

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PMID: 220 BattyMJChabrierGSheridanAGageMC