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Briefly, 60 L of recombinant GST- SERF2 at different concentrations (250 nM and 50 nM) in binding buffer (25 mM Tris-HCl pH 7.5, 150 mM KCl or LiCl, 3 mM MgCl 2 , 500 g/mL Ultrapure BSA and SUPERase-In RNase Inhibitor) were equilibrated with 60 L of pre-washed (binding buffer) magnetic beads (Dynabeads MyOne Streptavidin T1, Invitrogen) for 30 min at 4 C

1G) [37, 38], siRNA knockdown cells were not more sensitive to ferroptotic challenge (Supplementary Fig

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Nucleic Acids 35:102258