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22 The microbiota resort to the gut mucosal barrier during dietary fiber deficiency to obtain nutrients

When ROS increases, the synthesis of mitochondrial fusion-related proteins will be inhibited, thus promoting mitochondrial fission, which in turn may lead to more ROS production and severe mitochondrial dysfunction

doi: 10.1016/j.matdes.2023.112231 123 LoiseauA.AsilaV.Boitel-AullenG.LamM.SalmainM.BoujdayS

Determination of free iron content Senescent A549 cells were seeded in T75 cell culture flasks (2 10^6 cells per flask) and cultured for 24 h, and the nano-drugs were added to the corresponding cell cultures for another 4 h

In addition, a GSH/GSSG assay kit was used in this study, which is capable of accurately quantifying GSH/GSSG in cells by utilizing the enzymatic reaction between 5, 5-dithiobis-2-nitrobenzoic acid (DTNB) and glutathione reductase (GR)