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Preparation of NEM-derivatized cysteine and GSH internal standards The N-ethylmaleimide (NEM) derivatized isotope labeled [ 13 C 3 , 15 N]-cysteine-NEM and [ 13 C 2 , 15 N]-GSH-NEM were prepared by derivatizing the [ 13 C 3 , 15N]-cysteine and [ 13 C 2 , 15 N]-GSH standards with 50 mM NEM in 10 mM ammonium formate (pH = 7.0) at room temperature (30 min) as previously described 95

1: Investigations of our patient MCV: Mean corpuscular volume, MCH: Mean corpuscular hemoglobin, WBC: White blood cell, TSH: Thyroid stimulating hormone, LDH: Lactate dehydrogenase, DCT: Direct Coombs test, ANA: Anti-nuclear antibody, IF: Intrinsic factor, NCS: Nerve conduction study, SACD: Subacute combined degeneration of the spinal cord Fig

Known as the body's "master antioxidant," it plays a critical role in cellular defense by neutralizing oxidative stress and facilitating detoxification processes

Talukdar and Talukdar, 2014) and Pro (Omidi, via decreasing H 2 O 2 level and maintaining an increased level of reduced GSH and GSSG/GSH ratio

Polarizing macrophages in vitro