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TLR4 mediates activation of NADPH oxidase using myeloid differentiation factor 88 (MyD88), TLR4 TIR domain-associated protein (TIRAP) and Brutons tyrosine kinase (Btk)

The composite phylogeny supported several hypotheses from morphological literature: including Olixon as most genetically as well as morphologically distinct, the relationship of Rhopalosoma and Paniscomima as sister taxa, and the monophyly of all four genera

Cryosections (16-m thick) were permeabilised with 0.3% Triton X-100 in PBS for 2 min at 24 C, blocked with a blocking buffer composed of 5% BSA in PBS for 1 h at 24 C, and incubated with primary antibodies in a buffer composed of 1% BSA and 0.1% Triton X-100 in PBS for 16 h at 4 C