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Statements Conflict of interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest

Folate is critical for the development of the neural tube during the first six months of pregnancy and may help prevent neural tube defects such as spina bifida and anencephaly Folic acid helps make healthy red blood cells, which carry oxygen around the body

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Thrombin was removed by Benzamidine-Sepharose 6B resin (Cytiva) and tag-free TbTR was further purified by reverse immobilized metal affinity chromatography (Protino Ni-TED resin, Macherey-Nagel)

Key Research Applications : Appetite Suppression Research: Both peptides influence satiety centers in the brain, helping to model how hunger can be naturally reduced in response to hormonal signaling