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The isolated immunoprecipitates underwent a series of washes with cell lysis buffer (20 mM Tris, pH 8.0, 100 mM NaCl, 1 mM EDTA, and 0.5% NP-40) for five cycles, followed by three washes with TBS

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OCR was determined by six measurement cycles before and after injection: 3 min mixing, 3 min waiting and 3 min measuring

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