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As with all the dimorphic pathogenic fungi ( Blastomyces dermatitidis , Histoplasma capsulatum, Paracoccidioides brasiliensis and Sporothrix schenckii) the pathogenic form in tissue looks completely different form the saprobic mycelial form found in the environment

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After intensive wash, the GST-tagged proteins were cleaved off the beads with PreScission protease overnight at 4 C, and the His-tagged samples were eluted from the beads with lysis buffer supplemented with 250 mM imidazole

atp2 , complex V) on media containing glycerol or galactose as the main or sole carbon source (Fig