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After incubation, citrulline was quantified by colorimetry with diacetylmonoxime at 490 nm as previously described (25)

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labrax juveniles to mixtures of microplastics and mercury (another common contaminant of high concern found in different concentrations in the environment such as 0.5 to 200 ng/L in the North Sea 35 , 39 to 430 ng/L in the Wuli Estuary, China 36 , and 990 to 27,060 ng/L in the Mediterranean Sea 37 ) was found to reduce the swimming performance, cause neurotoxicity, and induce changes in the activity of energy-related enzymes 27,28