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Preferably, the binding specificity of monoclonal antibodies produced by the hybridoma cells is determined by immunoprecipitation or by an in vitro binding assay, such as radioimmunoassay (RIA) or enzyme-linked immunoadsorbant assay (ELISA)

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A washout interval of 2 to 4 weeks was maintained between each arm of the crossover to minimize potential carryover effects

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