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DR is a form of microangiopathy

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Recombinant Muc1 or lubricin mixed with one of the following enzymes: 8 unit/mL of proteinase K (P8107S, New England Biolabs), 500 g/mL of pronase (10165921001, Roche), 150 g/mL of trypsin (J63688.03, Thermo Scientific Chemicals), 20 g/mL of StcE, or 100 g/mL of papain (P4762, Sigma-Aldrich), were incubated at 37C for 2 h and analyzed by SDS-PAGE on a 3-8% tris-acete NuPAGE gel (Invitrogen) and stained with Pierce Silver Stain Kit (Thermo Scientific) according to the manufacturers instructions

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