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RAW264.7 Macrophage Cell Culture and LPS-Induced Nitric Oxide Production RAW264.7 cells, a murine macrophage cell line (IBMS, CAMS/PUMC, Beijing China), were cultured in Dulbeccos modified Eagles medium high glucose (Hyclone, Logan, UT, USA) medium with 10% fetal bovine serum (HyClone, Logan, UT, USA), 100 U/ml penicillin, and 100 g/ml streptomycin in a humidified atmosphere containing 5% CO 2 at 37C

doi: 10.3390/ijms21010295 97 WyseBAFuchs WeizmanNKadishSBalakierHSangaralingamMLibrachCL

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4 Fatigue Despite getting adequate sleep, individuals may feel constantly tired and lacking energy for daily activities