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Quantitative PCR was performed using PowerUp SYBR Green Master Mix (Applied Biosystems, #A25742) in 10 L reactions containing 5 L master mix, 0.5 M of each primer, and 20 ng cDNA template

doi: 10.1136/ard.2004.030528 253 RiccardoLLClaudiaCIreneMAntonioGAlfredoP

This percentage was similar to the 16% prevalence of depression symptoms that was reported in students from the beginning to end of college (Romo-Nava et al., 2019), the severity of which was mild to moderate (Rosas-Santiago et al., 2016)

42 For example, extract of sulforaphane, a sulfur-rich compound found in broccoli, has been shown to increase nuclear factor erythroid-2-related factor 2 (Nrf2) and its regulated antioxidants in upper airways, and to improve the ability of macrophages to remove bacteria

After undergoing magnetic stirring for 8 h at room temperature, mPEG 5000 -OH (0.68 g, 0.13 mmol) was introduced into the reaction system