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In another significant study, the effects of sodium butyrate on pancreatic development in dairy calves over a 70-day period were examined [31]

Khodaparast, M

doi: 10.1089/ars.2014.5851 32 Espinosa-DiezCMiguelVMennerichDKietzmannTSnchez-PrezPCadenasSet al

For preparation of the membrane fraction the pellet was washed in 5 mL of 50 mM MES-KOH, 20 mM KCl, pH 6.5, centrifuged (150,000 g , 60 min at 4 C), resuspended in 500 L of the same buffer and finally sonicated (20%, 0.5 s 1 , on ice) until membranes were fully suspended

Tabe Y, Jin L, Kazuno S, Fujimura T, Matsushit H, Ueno T, Miida T, Andreeff M , Gutterman JU, Konopleva M