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4 Discussion Selenium, iron, and vitamin E intake were increased in supplemented animals throughout the experimental period and changes in the concentrations of blood metabolites of animals were observed, indicating their supportive role against pathogens

Turk J Biol 36:8592 Pillco A (2011) Evaluacin toxicolgica de mezclas complejas mediante ensayos alternativos a la experimentacin animal de genotoxicidad, mutagenicidad y ecotoxicidad (Toxicological evaluation of complex mixtures employing genotoxicity, mutagenicity and ecotoxicity assays as an alternative to animal testing) Doctoral thesis, Autonomous University of Madrid, p 173 Rank J, Nielsen MH (1998) Genotoxicity testing of wastewater sludge using the Allium cepa anaphase-telophase chromosome aberration assay

Since stress-responsive cis -acting elements existed in the promoters of the RcGPXs , qRT-PCR was performed to investigate the expression change of RcGPXs under cold, drought, and salt stresses (Figure 4)

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