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qPCR analysis qPCR for selected wheat genes was conducted with the iQ SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA) on a BioRad-iCycler detection system as described previously (Liu et al., 2010)

Altered expression of Cu transporters in several cancers has been also associated with elevated Cu supply, fueling different processes that support tumor growth 9

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Glutathione plays a key role in reducing melanin production, the pigment responsible for dark spots and hyperpigmentation

Then, the NPT ensemble was further employed for 50 ns to equilibrate the system at 300 K and 1 atm, followed by a 10 ns production run for collecting equilibrated configurations at each 10 ps interval