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In: Arya A, Rusevska K (eds) Biology, cultivation and applications of mushrooms, 1st edn

Colonies were screened by sanger sequencing to ensure proper orientation and copy number of the insert

10.1016/j.wneu.2015.09.052 61 GuoS.XingN.XiangG.ZhangY.WangS

Cells were fixed in 4% (v/v) paraformaldehyde (PFA) (Pierce, 28906) in PBS for 20 min and permeabilised in 0.1% (v/v) Triton X-100 in PBS (Sigma-Aldrich), or in 0.1% (w/v) saponin (sigma-Aldrich) in PBS when labelling LAMP1 compartments, for 5 min followed by blocking with 2% (w/v) BSA (Sigma, 05482) in PBS for 30 min

Gsrs across phyla share a common ancestor, suggesting that this enzyme arose only once [50]