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However, the degradation of l-cysteine was not eliminated completely due to the activities of other cysteine desulfhydrases in E

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, GRX1-roGFP2 or TRaQ-G) enable real-time, organelle-specific redox readouts but primarily report redox potential rather than absolute GSH levels, whereas fractionation coupled with mass spectrometry provides absolute GSH/GSSG quantification but remains static and susceptible to lysis-associated artifacts

The GST and GPX activities were higher in the auxin autotrophic tobacco callus lines which were suggested to enhance salt stress (100 mmol/L NaCl) tolerance