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The fragment size of PCR amplified products was confirmed by agarose gel electrophoresis on 1.5% Agarose gel against 100 bp (bp) DNA ladder (Thermo Fisher Scientific, USA)

Interestingly, the early (3.5 days) slight (0.5-fold more than controls, while the peak was 7-fold) but significant increase in Enpp6 -positive newly-forming/formed OLs was accompanied by a concomitant and very comparable degree of decrease (52%) in Pdgfra -positive OPCs, with the specific values of 50 per mm 2 increase for Ennp6 -positive cells, and 80 per mm 2 decrease for Pdgfra -positive cells

May cause hypokalemia during initial treatment (monitor potassium) 3

Bartolo, Sensors 21, (2021)

CLAs, as a nutritional supplement, have gained popularity among athletes due to their pleotropic positive effects on human health [12]