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Separation was achieved using a Waters Acquity UPLC BEH C18 column (1.7 m particle size, 2.1 mm 50 mm), maintained at 40 C

Upon TNF stimulation, NF-B and MAPK pathways are activated to promote the transcription of proinflammatory and pro-survival genes 43

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Juang et al., 2014)

At 7 days in vitro, cells in each well were infected with 5 l AAV vector (titre: 1 10 11 vg/ml) to express STAND proteins in a neuron-specific manner by a synapsin promoter and analysed by immunocytochemistry or used for the DA release assay 7 days later