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It was next verified whether Grx1 plays an important role in acute lung injury by regulating S-glutathionylation levels in macrophages

E., Penaloza, A., Zahir, S

In order to exclude any virus-dependent effects, we further investigated the T cell-dependent (TD) response in Gclc fl/fl Mb1-Cre + mice using a stronger, T cell-driven model of cytopathic viral infection

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GSH or Ni-NTA bead washing was performed four times with cold PBS or washing buffer (20 mM Tris-Cl (pH 8.0), 300 mM NaCl, 20 mM imidazole)