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Also, vitamin C infusion is contraindicated in individuals with certain conditions (for example, those with G6PD deficiency)

Nevertheless, at high concentrations, and in the presence of catalytic metal ions such as ferric iron (Fe 3+ ), VC can also have a pro-oxidative behavior through an indirect contribution in the Fenton reaction [6]

For SDS-gel electrophoresis proteins were isolated from four to eight cervical spinal cord samples (p40) per genotype using Cell lysis buffer (#9803S, Cell Signaling Technology, Danvers, Massachusetts, USA) supplemented with protease inhibitor (#11697498001, Merck, Darmstadt, Germany)

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The membrane was blocked with PBS containing 0.5% tween (PBST) and 5% milk, and probed overnight with affinity-purified anti-GspD or anti-PilQ in PBST plus 3% BSA