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Basal insulin was measured in plasma from female [Young ( n = 16), Old Con ( n = 26), and Old mAb ( n = 25)] and male mice [Young ( n = 17), Old Con ( n = 32), and Old mAb ( n = 28)] using a bead-based assay for mouse insulin (EMD Millipore, Inc) and detection was performed on a Bio-Plex MAGPIX Multiplex Reader (Biorad Inc., Hercules, CA)

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2 Further investigation revealed that this type of cell death does not share classic features of apoptosis such as caspase activation and chromatin fragmentation and is characterized by the iron-dependent accumulation of lipid hydroperoxides to lethal levels

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doi: 10.1016/S0190-9622(99)70190-8