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The cells in a 96-well plate were incubated in HBSS containing 10 mM FAO blue for 2 h, washed twice with HBSS, and measured using a Spectramax M2 plate reader (excitation/emission = 405 nm/465 nm)

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Mullikin D, Pillai N, Sanchez R et al (2018) Megaloblastic anemia progressing to severe thrombotic microangiopathy in patients with disordered vitamin B12 metabolism: case reports and literature review

U-2 OS FSP1 GFP-P2A-BFP /RFK KO cells stably expressing Cas9 were transduced with the packaged library as described above

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