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doi: 10.17532/jhsci.2019.737 96 Rubio-AliagaI.WagnerC

For siRNA-mediated gene silencing, M0-human macrophages or BMDMs were transfected with 25 nM of Silencer pre-designed siRNA targeting human LIPE /HSL (143993 and 143994, Life Technologies), Lipe mouse pre-designed siRNA Set A (HY-RS18621, MCE), or a non-targeting control pool (Dharmacon, Lafayette, CO) using Lipofectamine LTX Transfection Reagent (Thermo Fisher Scientific)