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Use 2 vials of Substrate for a 96-well microplate (total volume: 7.2 ml)

This pathway relies on -catenin, which is activated by binding extracellular Wnt ligands to membrane receptors through autocrine and paracrine processes, inhibiting the degradation of -catenin so that it can be stably accumulated in the cytoplasm and transferred to the nuclear compartment, where it can work together with T cell factor/lymphoenhancer binding factor to stimulate the transcription of target genes

ClinialTrial.gov [online], (2013)

Woo JH, Choi YS, Choi JH

Fu X, Kao JLF, Bergt C, Kassim SY, Huq NP, Avignon A, Parks WC, Mecham RP, Heinecke JW (2004) Oxidative cross-linking of tryptophan to glycine restrains matrix metalloproteinase activity: specific structural motifs control protein oxidation