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Identification of the NF1 mutant allele in BC Patient 1-derived hiPSCs To validate the genetic background of the NF1 mutation in BC patient-derived hiPSCs compared to BC tissues, genomic DNA was extracted from normal and patient-derived hiPSCs using the DNeasy Blood & Tissue Kit (QIAGEN, #69504, Hilden, Germany) according to the manufacturers instructions

Physiol Rev (1983) 63:547605.10.1152/physrev.1983.63.2.547 99 NewsholmeEACrabtreeBArdawiMS

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S10B), indicating that GSK3 phosphorylation in Ssu72-deficient adipocytes is largely independent of AKT activity

Recently, a lot of evidence has shown that H 2 S has a double-edged sword effect in HCC, but the mechanism is not fully understood