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Biological activity of autoantibodies from patients with thyroid-associated ophthalmopathy: in vitro effects on porcine extraocular myoblasts

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The supernatant was transferred to a deep-well 96-well plate, which then was centrifuged for an additional 10 minutes at 2000 x g and 4C to ensure that any precipitate that may have been transferred to the deep-well plate was forced to the bottom of the wells

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Interestingly, this effect was independent of patients sex, MGMT methylation, p53 mutations or EGFR status