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In brief, enzyme conjugate (200 l) was added to 25 l of sample and standard (0, 0.2, 0.5, 1, 2, 6, and 16 ng/ml) well, mixed and incubated at room temperature (RT) for one hour

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Yoshino M, et al

Double-blind, randomized trial comparing the efficacy and tolerability of fulvestrant versus anastrozole in postmenopausal women with advanced breast cancer progressing on prior endocrine therapy: results of a North American trial