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doi:10.1038/s41477-018-0339-y

Differential gene expression in giardia lamblia under oxidative stress: Significance in eukaryotic evolution

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To assess the survival of bacteria detected by the reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH) by the addition of DTT, as well as to scavenge ROS by the addition of thiourea and to chelate divalent iron ions by the addition of 2,2-bipyridyl, the strains were treated using the same methodology described above: 10 mM DTT or 500 M 2,2-bipyridyl was added to the medium, the mixture was incubated at 37C for 8 h, diluted evenly on soybean casein agar medium, incubated overnight, and the number of CFUs was counted

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