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Then, the supernatant containing the cell-free culture media was transferred to a new tube, and 50 mL of the Total Exosome Isolation reagent (Cat

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10.1016/j.scitotenv.2022.160032 62 DineshR.SreenaC

These radicals attack polyunsaturated fatty acids (PUFAs) in the cell membrane, initiating a lipid peroxidation chain reaction, membrane disruption, and ferroptosis ( 3.1.2 Lipid peroxidation Lipid peroxidation is one of the essential factors driving ferroptosis and serving as a major cause of cell death (Figure 2) (Rochette et al., 2022)