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After 24 h, cells were washed, suspended in 1 PBS, and stained with 3 M PI for 15 min in the dark

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Despite some similarities such as promoting reduced body weight [13], substantial differences between CR and IF have been uncovered [5] and are, indeed, expected, since CR involves a daily reduction in food intake, while IF changes feeding frequency, and may not always result in a limitation of food ingestion [7, 14]