FIGURE 4 3.5 Determination of the main constituents from Wuling capsule and its drug-containing serum As shown in Figures 5AD, forty-one compounds were determined and identified in Wuling capsule based on the relative retention time and evaluation of MS data acquired in both positive and negative ion modes (Supplementary Table S1)
Blood (10 l each) was collected in 200 l of CLB buffer (PBS, 20 mM HEPES, 20 mM Glucose, 4 mM sodium bicarbonate, 1 mM EDTA, 0.1% w/v bovine serum albumin, pH 7.25)
Thiopurine Enhanced ALL Maintenance (TEAM): study protocol for a randomized study to evaluate the improvement in disease-free survival by adding very low dose 6-thioguanine to 6-mercaptopurine/methotrexate-based maintenance therapy in pediatric and adult patients (0-45 years) with newly diagnosed B-cell precursor or T-cell acute lymphoblastic leukemia treated according to the intermediate risk-high group of the ALLTogether1 protocol
DNA and RNA isolation Genomic DNA (gDNA) was extracted from blood leukocytes or cultured dermal fibroblasts using the PureGene DNA extraction method according to the manufacturers instructions (Gentra Systems, Minneapolis, MN)
The exposure of oral tissues to environmental hazards is immense, especially in smokers, and acrolein was shown to reduce GSH, and increase oxidative stress and protein carbonylation in oral keratinocytes in vitro ( Avezov et al