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After several washes in the same buffer, the samples were post-fixed for 60 minutes with 2% osmium tetroxide, washed in deionised water, dehydrated through graded ethanol (70, 95, and 100%) and embedded in epon for 48 hours at 60 C

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qRT-PCR analysis Raw 264.7 cells were seeded in a 6-well plate (3 10 5 cells per well) and stimulated with LPS (10 g/mL) for 24 h