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Hair and nail germ formation and elongation assay For the formation and elongation assay of hair and nail germs, primary skin dermal fibroblasts, skin epidermal keratinocytes, hair follicle dermal papilla cells, and nail fibroblasts (onychofibroblasts) were obtained from patients under ethical considerations

This has been experimentally demonstrated by silencing the estrogen receptor in breast cancer (ER+) with consequent decrease of OCTN2 expression (Wang et al., 2012)

Exfoliate Once or Twice a Week Use a chemical exfoliant (BHA/AHA) instead of harsh scrubs

Subedi et al., 2019

Application frequency for topical protocols is typically once or twice daily, applied to cleansed skin at the target area